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iPr-Pac-G-CE Phosphoramidite - (10-3021)

5'-Dimethoxytrityl-N-p-isopropylphenoxyacetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3021

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Product Specifications

Formula:

C₅₇H₇₄N₇O₁₀PSi

M.W.:

1076.31

F.W.:

345.21

CAS Number:

689283-85-0

5'-Dimethoxytrityl-N-p-isopropylphenoxyacetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certi cate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling ef ciency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3021-02	0.25 g	.25grams	2.32	64	38.4	24	17.45	12.8	3.2
10-3021-05	0.5 g	.5grams	4.65	141.67	85	53.13	38.64	28.33	7.08
10-3021-10	1.0 g	1grams	9.29	296.33	177.8	111.13	80.82	59.27	14.82

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3021-02	0.25 g	.25grams	3.47	0.07	63	39.3	13.13	3.9
10-3021-05	0.5 g	.5grams	6.93	0.07	132.2	82.6	27.54	8.2
10-3021-10	1.0 g	1grams	13.87	0.07	271	169.3	56.46	16.9