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2'-OMe-iPr-Pac-G-CE Phosphoramidite - (10-3621)

5'-Dimethoxytrityl-N2-isopropylphenoxyacetyl-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3621

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Product Specifications

Formula:

C₅₂H₆₂N₇O₁₀P

M.W.:

976.07

F.W.:

359.24

CAS Number:

1402072-31-4

5'-Dimethoxytrityl-N2-isopropylphenoxyacetyl-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

The use of UltraMild monomers in oligonucleotide synthesis has allowed very sensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely. The DNA and RNA monomers are currently available and we also provide this set of 2'-OMe-RNA monomers. In our version of this chemistry, we use as protecting groups phenoxyacetyl (Pac) for A, acetyl (Ac) for C, and isopropyl-phenoxyacetyl (iPr-Pac) for G.

It has become clear that acetic anhydride in the conventional capping mix can cause transamidation in situations where an amine protecting group is quite labile. This leads to acetyl protection on the amino group that may be slow to be removed. Consequently, if many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix.

Details

Usage

Coupling: 6 minute coupling time. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMILD 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3621-02	0.25 g	.25grams	2.56	72	43.2	27	19.64	14.4	3.6
10-3621-05	0.5 g	.5grams	5.12	157.33	94.4	59	42.91	31.47	7.87
10-3621-10	1.0 g	1grams	10.25	328.33	197	123.13	89.55	65.67	16.42
10-3621-90	100 µmol	.098grams	1	20	12	7.5	5.45	4	1

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3621-02	0.25 g	.25grams	3.82	0.07	70	43.75	31.82	4.38
10-3621-05	0.5 g	.5grams	7.65	0.07	146.6	91.63	66.64	9.16
10-3621-10	1.0 g	1grams	15.29	0.07	299.4	187.13	136.09	18.71
10-3621-90	100 µmol	.098grams	1.5	0.07	23.6	14.75	10.73	1.48