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4-Thio-U-TOM-CE Phosphoramidite
5'-Dimethoxytrityl-4-(2-cyanoethylthio)-Uridine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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Description
Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.
Details
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
Catalog #
Pack Size
Grams/Pack
0.1M Dil. (mL)
Approximate Number of Additions
LV40
LV200
40nm
0.2μm
1μm
10μm
10-3052-02
0.25 g
.25grams
2.49
69.67
41.8
26.13
19
13.93
3.48
10-3052-90
100 µmol
.1grams
1
20
12
7.5
5.45
4
1
10-3052-95
50 µmol
.05grams
0.5
3.33
2
1.25
0.91
0.67
0.17
Expedite
Catalog #
Pack Size
Grams/Pack
Dilution (mL)
Approximate Number of Additions
Molarity
50nm
0.2μm
1μm
15μm
10-3052-02
0.25 g
.25grams
3.72
0.07
68
42.5
14.17
4.2
10-3052-90
100 µmol
.1grams
1.5
0.07
23.6
14.7
4.92
1.4
10-3052-95
50 µmol
.05grams
0.75
0.07
8.6
5.3
1.79
0.5
