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U-CE Phosphoramidite - (10-3030)

5'-Dimethoxytrityl-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3030

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Product Specifications

Formula:

C₄₅H₆₁N₄O₉PSi

M.W.:

861.06

F.W.:

306.17

CAS Number:

118362-03-1

5'-Dimethoxytrityl-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minute coupling time recommended
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3030-02	0.25 g	.25grams	2.9	83.33	50	31.25	22.73	16.67	4.17
10-3030-05	0.5 g	.5grams	5.81	180.33	108.2	67.63	49.18	36.07	9.02
10-3030-10	1.0 g	1grams	11.61	373.67	224.2	140.13	101.91	74.73	18.68

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3030-02	0.25 g	.25grams	4.33	0.07	80.2	50.13	16.71	5.01
10-3030-05	0.5 g	.5grams	8.67	0.07	167	104.38	34.79	10.44
10-3030-10	1.0 g	1grams	17.33	0.07	340.2	212.63	70.88	21.26