Over the years Glen Research. has provided a large nunber of unusual monomers for oligohuclebtide synthesis. In this issue,.we have described several under specific headings. As usual though, we haye a couple of items that defy being placed in an obvious category- unusuaLmonomers.
Adenosine and Cytosine Arabinosides have been used as antiviral and anti-leukemia drugs, respecitvely. Ara-C is of special interest because of its effect as an inhibitor of DNA replication. It is desirable, therefore, to incorporate Ara-C into specific sites in DNA to investigate the mode of activity. Ara-C-CE Phosphoramidite1 is designed for this purpose. Methylation of DNA occurs predominantly at the O6 position of dG and the O4 position of dT. Oligonucleotides containing O5-Me-dG can be prepared using the posphoramidite or in situ (see Literature Reveiw on Page 5). We are now able to offer O4-Me-dT-CE Phosphoramidite also.
2) G.P. Beardsley, T. Mikita, . M.M. Klaus, and AL. Nussbaum, Nucleic Acids Res., 1988, 16, 9165.
O4-Me-dT-CE Phosphoramidite (10-1032) has been discontinued
Ara-C-CE Phosphoramidite (10-4010) has been discontinued.