Home Products Modification and Labeling Label Phosphoramidites and Supports Cholesterol Labeling of Oligonucleotides Cholesteryl-TEG Phosphoramidite

Cholesteryl-TEG Phosphoramidite - (10-1975)

1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol-glyceryl-2-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

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10-1975

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Product Specifications

Formula:

C₇₀H₁₀₆N₃O₁₁P

M.W.:

1196.6

F.W.:

755.97

CAS Number:

873435-29-1

1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol-glyceryl-2-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

Description

Potential therapeutic oligonucleotides must permeate the cell membrane for optimal activity. The addition of lipophilic groups to an oligonucleotide would be expected to enhance cellular uptake/membrane permeation. The use of Cholesterol-labelled oligos and the consequent improvement in activity has been described. We have designed our Cholesteryl products with triethyleneglycol (TEG) spacers for maximum solubility.

Details

Usage

Coupling: 15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on.
Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.

Specifications
Diluent	10% THF in Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1975-02	0.25 g	.25grams	2.09	56.33	33.8	21.13	15.36	11.27	2.82
10-1975-90	100 µmol	.12grams	1	20	12	7.5	5.45	4	1
10-1975-95	50 µmol	.06grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1975-02	0.25 g	.25grams	3.12	0.07	56	35	25.45	3.5
10-1975-90	100 µmol	.12grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1975-95	50 µmol	.06grams	0.75	0.07	8.6	5.38	3.91	0.54