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5-Me-C-TOM-CE Phosphoramidite - (10-3064)

5'-Dimethoxytrityl-N-acetyl-5-methyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3064

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	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₅₂H₇₄N₅O₁₀PSi

M.W.:

988.25

F.W.:

319.21

CAS Number:

872548-65-7

5'-Dimethoxytrityl-N-acetyl-5-methyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Details

Usage

Coupling: See technical bulletin for details.
Deprotection: See technical bulletin for details.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3064-02	0.25 g	.25grams	2.53	71	42.6	26.63	19.36	14.2	3.55
10-3064-90	100 µmol	.099grams	1	20	12	7.5	5.45	4	1
10-3064-95	50 µmol	.049grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3064-02	0.25 g	.25grams	3.78	0.07	69.2	43.2	14.42	4.3
10-3064-90	100 µmol	.099grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3064-95	50 µmol	.049grams	0.75	0.07	8.6	5.3	1.79	0.5