Amino-Modifier C6-U Phosphoramidite

5'-Dimethoxytrityl-5-[N-(trifluoroacetylaminohexyl)-3-acrylimido]-Uridine, 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:

C₆₀H₈₄F₃N₆O₁₂PSi

M.W.:

1197.41

F.W.:

474.40

CAS Number:

210534-16-0

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Technical Documents

Description

Details

Dilution/Coupling Data

Technical Documents

Glen Report 17.11: Introduction

Glen Report 17.12: Products for siRNA Research

Glen Report 19.11: Modified RNA Phosphoramidites Useful in siRNA Research and Biologically Significant 1-Methyl-adenosine

Description

Amino-Modifier C6-U has been added to the growing family of sequence modifiers and we envisage applications in RNA structural studies as well as for labelling siRNA to probe uptake and cellular distribution.

Details

Usage

Coupling: 12 minute coupling time recommended
Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30�C, dry
Stability	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3039-02	0.25 g	.25grams	2.09	56.33	33.8	21.13	15.36	11.27	2.82
10-3039-90	100 µmol	.12grams	1	20	12	7.5	5.45	4	1
10-3039-95	50 µmol	.06grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3039-02	0.25 g	.25grams	3.12	0.07	56	35	11.67	3.5
10-3039-90	100 µmol	.12grams	1.5	0.07	26.6	14.7	4.92	1.4
10-3039-95	50 µmol	.06grams	0.75	0.07	8.6	5.3	1.79	0.5