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Zebularine-CE Phosphoramidite - (10-3011)

5'-Dimethoxytrityl-pyrimidin-2-one-riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3011

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Product Specifications

Formula:

C₄₅H₆₁N₄O₈PSi

M.W.:

845.05

F.W.:

290.17

CAS Number:

155831-90-6

5'-Dimethoxytrityl-pyrimidin-2-one-riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Ribozyme activity is substantially affected by the substitution of modified pyrimidine bases. Zebularine (pyrimidin-2-one ribonucleoside) may be regarded as a Cytidine derivative lacking the exocyclic amino group. Zebularine and Pyridin-2-one Ribonucleoside, the 3-deaza analogue of Zebularine, are prime candidates for use in evaluating ribozyme activity and function. It should be noted that Zebularine is mildly fluorescent, absorbing at 298nm and emitting at 367nm.

Details

Usage

Coupling: 12 minute coupling time is recommended. Monomers that allow for UltraMild deprotection must be used. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: Cleave and remove base protection in 30% Ammonium Hydroxide:EtOH (3:1) for 4 hours at Room Temperature. Complete 2' deprotection per RNA Technical Bulletin. |See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3011-02	0.25 g	.25grams	2.96	85.33	51.2	32	23.27	17.07	4.27
10-3011-90	100 µmol	.085grams	1	20	12	7.5	5.45	4	1
10-3011-95	50 µmol	.042grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3011-02	0.25 g	.25grams	4.42	0.07	82	61.2	17	5.1
10-3011-90	100 µmol	.085grams	1.5	0.07	23.6	14.7	4.9	1.4
10-3011-95	50 µmol	.042grams	0.75	0.07	8.6	5.3	1.7	0.5