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5-Me-2'-deoxyZebularine-CE Phosphoramidite - (10-1061)

5'-Dimethoxytrityl-5-methyl-pyrimidin-2-one-2'-deoxyriboside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1061

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Product Specifications

Formula:

C₄₀H₄₉N₄O₇P

M.W.:

728.82

F.W.:

288.19

CAS Number:

125258-62-0

5'-Dimethoxytrityl-5-methyl-pyrimidin-2-one-2'-deoxyriboside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Zebularine (pyrimidin-2-one ribonucleoside) is a cytidine analogue that acts as a DNA demethylase inhibitor, as well as a cytidine deaminase inhibitor. This structure is very active biologically and Zebularine is now used as a potent anti-cancer drug. A 2'-deoxynucleoside analogue of Zebularine, 5-methyl-pyrimidin-2-one, 2'-deoxynucleoside, has been used to probe the initiation of the cellular DNA repair process by making use of its mildly fluorescent properties. This combination of biological activity and fluorescence properties would make 5-Me-2'-deoxyZebularine a strong addition to our array of nucleoside analogues.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Ammonium Hydroxide for 24 hrs at room temperature or as required by nucleobases.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1061-02	0.25 g	.25grams	3.43	101	60.6	37.88	27.55	20.2	5.05
10-1061-90	100 µmol	.073grams	1	20	12	7.5	5.45	4	1
10-1061-95	50 µmol	.036grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1061-02	0.25 g	.25grams	5.12	0.07	96	60	43.64	6
10-1061-90	100 µmol	.073grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1061-95	50 µmol	.036grams	0.75	0.07	8.6	5.38	3.91	0.54