Trimer Phosphoramidite Mix 1 - (13-1991)
Mix of 20 Trimers(AAA/AAC/ACT/ATC/ATG/CAG/CAT/CCG/CGT/CTG/GAA/GAC/GCT/GGT/GTT/TAC/TCT/TGC/TGG/TTC)(5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0)
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13-1991
Mix of 20 Trimers(AAA/AAC/ACT/ATC/ATG/CAG/CAT/CCG/CGT/CTG/GAA/GAC/GCT/GGT/GTT/TAC/TCT/TGC/TGG/TTC)(5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0:5.0)
Description
All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys). For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact support@glenresearch.com for a quotation and projected delivery date.
Details
Usage
- Coupling: 15 minute coupling time recommended.
- Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 13-1991-90 | 100 µmol | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 | |
| 13-1991-95 | 50 µmol | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 | |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 13-1991-90 | 100 µmol | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 13-1991-95 | 50 µmol | 0.75 | 0.7 | 8.6 | 5.38 | 3.91 | 0.54 | |
Technical documents
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CAG Trimer Phosphoramidite
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