Due to their versatility, it’s no surprise that universal supports remain popular over requiring a support for each 3’-nucleoside or modification. Particularly when a special modification is only available as a phosphoramidite, a reliable universal support is essential. Our UnySupport, which is based on UnyLinker chemistry, was first introduced in 2008 (Figure 1).1 Since then, we have included multiple versions: 500Å CPG, 1000Å CPG, high load CPG, and polystyrene.2 We are pleased to introduce two new versions of UnySupport: 1400Å CPG and 2000Å CPG.
Figure 1. UnySupport Structure
Prior to now, our 2000Å CPG supports were limited to main DNA bases (A, C, G, and T). The introduction of UnySupport 2000Å CPG allows researchers to use all of our modifiers in a 2000Å environment. The 2000Å supports are best for very long (>150 mer) oligonucleotides. However, a caveat to using such a large pore size is the limited loading. For customers who do not wish to sacrifice this, we are also offering UnySupport 1400Å CPG, which is capable of synthesizing long oligonucleotides without compromising loading.
Oligonucleotide deprotection and cleavage from Glen UnySupport can be carried out with various methods: UltraFast, Standard, UltraMild, or gas phase. The cleavage mechanism is two steps and has been described in previous Glen Reports.1 Deprotection with Ammonium Hydroxide:MethylAmine (AMA) 1:1 requires 1 hour at 65 °C or Ammonium Hydroxide for 8 hours at 55 °C. For sensitive minor bases or dyes, Glen UnySupport may be eliminated with 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60 °C. Glen UnySupport is also compatible with Methylamine gas for 30 minutes at 65 °C at 30 psi.
References
The Glen Report, 2008, 20.2, 10-11.
The Glen Report, 2009, 21.1, 14.
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