5'-AquaPhluor® 639 CE Phosphoramidite - (10-5926)
6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H- Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6- (N-hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl] [trifluoroacetylaminobutoxy]phosphoryl][oxy]-hexyl-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
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10-5926
6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H-
Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6-
(N-hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl]
[trifluoroacetylaminobutoxy]phosphoryl][oxy]-hexyl-1-O-
(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Description
AquaPhluor® 639 is a rhodamine dye with an excitation and emission in the far red of the visible spectrum and is a logical alternative to Cyanine 5. Like Cyanine 5, AP639 is relatively bright and exhibits sequence- and pH-independent fluorescence. Unlike Cyanine 5, AP639 also shows excellent chemical and photochemical stability as well as temperature-independent fluorescence. AP639 is available as the phosphoramidite and CPG for 5’ and 3’ labeling, respectively.
Details
Usage
- Coupling: 3 minute coupling time recommended
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-5926-95 | 50 µmol | 0.06 | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
| 10-5926-90 | 100 µmol | 0.13 | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5926-02 | 0.25 g | 0.25 | 1.96 | 52 | 31.2 | 19.5 | 14.18 | 10.4 | 2.6 |
Technical documents
Certificate of analysis
CoA search tool
Intellectual Property
Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. For additional information, please contact ELITechGroup. AquaPhluor® is a registered trademark of ELITechGroup.
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