Home Products DNA & RNA Nucleosides, Analogs, and Supports Nucleoside Analog Phosphoramidites DNA Nucleoside Analogs Analog Phosphoramidites for Structural Studies Analog Phosphoramidites for Structure/Activity Relationship of Oligonucleotides dX-CE Phosphoramidite

dX-CE Phosphoramidite - (10-1537)

5'-(4,4'-Dimethoxytrityl)-2,6-O-bis(2-(4-nitrophenyl)ethyl)-2'-deoxyXanthosine,3’-[(2-cyanoethyl)-N,N-diisopropyl)]-phosphoramidite

As low as $225.00

In stock

Only %1 left

default

10-1537

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₅₆H₆₁N₈O₁₂P

M.W.:

1069.1

F.W.:

330.19

CAS Number:

292050-43-2

5'-(4,4'-Dimethoxytrityl)-2,6-O-bis(2-(4-nitrophenyl)ethyl)-2'-deoxyXanthosine,3’-[(2-cyanoethyl)-N,N-diisopropyl)]-phosphoramidite

Description

2'-DeoxyXanthosine (dX) is a naturally occurring nucleoside that may be derived from oxidative deamination of 2'-deoxyGuanosine (dG). dX has a similar bonding pattern to thymidine and it may base pair with dA, with such purine-purine interactions causing duplex distortion. dX also featured in attempts to extend the genetic alphabet with a new base pair of dX and pyrimidine-2,4-diamine nucleoside. dX has also interested researchers in the field of DNA damage and repair since it is a product of nitric oxide-induced mutagenesis.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: With the oligonucleotide still attached to the support, treat with 1 M DBU in anhydrous ACN for 2-3 minutes, followed by 17 hours using fresh 1 M DBU at room temperature. Rinse support with ACN and then deprotect as required by nucleobases. Note, the dX nucleoside is susceptible to depurination though short exposure to acid is tolerated - e.g. Glen-Pak purification.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1537-02	0.25 g	.25grams	2.34	64.67	38.8	24.25	17.64	12.93	3.23
10-1537-90	100 µmol	.107grams	1	20	12	7.5	5.45	4	1
10-1537-95	50 µmol	.053grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1537-02	0.25 g	.25grams	3.49	0.07	63.4	39.63	28.82	3.96
10-1537-90	100 µmol	.107grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1537-95	50 µmol	.053grams	0.75	0.07	8.6	5.38	3.91	0.54