8-Amino-dG-CE Phosphoramidite - (10-1079)
5'-Dimethoxytrityl-N2,N8-bis(dimethylaminomethylidine)-8-amino-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
As low as
$215.00
In stock
Only %1 left
default
10-1079
5'-Dimethoxytrityl-N2,N8-bis(dimethylaminomethylidine)-8-amino-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Description
8-Amino-G is formed along with 8-oxo-G as the major mutagenic lesions formed in DNA damage caused by 2-nitropropane. 2-Nitropropane is an industrial solvent and a component of paints, dyes and varnishes, and is also present in cigarette smoke.
8-Amino-dA and 8-amino-dG are useful in triplex formation due to the presence of the additional amino groups.
Details
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.1M 2-mercaptoethanol, (7µl/ml) at 55° for 20 hours.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability In Solution | 2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1079-02 | 0.25 g | .25grams | 2.79 | 79.67 | 47.8 | 29.88 | 21.73 | 15.93 | 3.98 |
| 10-1079-90 | 100 µmol | .09grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1079-95 | 50 µmol | .045grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1079-02 | 0.25 g | .25grams | 4.17 | 0.07 | 77 | 48.13 | 35 | 4.81 |
| 10-1079-90 | 100 µmol | .09grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1079-95 | 50 µmol | .045grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
Technical documents
Certificate of analysis
CoA search tool
Related products
Related Products
8-Amino-dA-CE Phosphoramidite
Cat. No.: 10-1086
Thymidine Glycol CE Phosphoramidite
Cat. No.: 10-1096
dX-CE Phosphoramidite
Cat. No.: 10-1537
Abasic II Phosphoramidite
Cat. No.: 10-1927
8-Br-dA-CE Phosphoramidite
Cat. No.: 10-1007
References
To request a quote for products:
- Click “Contact Us” in the header bar above;
- Click “Customer Service”;
- Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
- Click “Submit”.