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6-Thio-G-CE Phosphoramidite - (10-3072)

5'-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3072

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Product Specifications

Formula:

C₅₃H₇₁N₈O₈PSSi

M.W.:

1039.31

F.W.:

361.26

5'-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used.

Details

Usage

Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015).
Deprotection: Treat column with 1M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3072-02	0.25 g	.25grams	2.41	67	40.2	25.13	18.27	13.4	3.35
10-3072-90	100 µmol	.104grams	1	20	12	7.5	5.45	4	1
10-3072-95	50 µmol	.052grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3072-02	0.25 g	.25grams	3.59	0.07	65.4	40.8	13.63	4
10-3072-90	100 µmol	.104grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3072-95	50 µmol	.052grams	0.75	0.07	8.6	5.3	1.79	0.5