5'-Bromohexyl Phosphoramidite - (10-1946)
6-Bromo-hex-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
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10-1946
6-Bromo-hex-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Description
Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2
Details
Usage
- Coupling: 3 minute coupling recommended.
- Deprotection: See Technical Bulletin for details (https://www.glenresearch.com/media//folio3/productattachments/technical_bulletin/TB_10-1946.pdf).
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 1-2 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1946-02 | 0.25 g | .25grams | 6.56 | 205.33 | 123.2 | 77 | 56 | 41.07 | 10.27 |
| 10-1946-90 | 100 µmol | .038grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1946-02 | 0.25 g | .25grams | 9.79 | 0.07 | 189.4 | 118.38 | 86.09 | 11.84 |
| 10-1946-90 | 100 µmol | .038grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
Technical documents
Certificate of analysis
CoA search tool
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