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Alkyne-Modifier Serinol Phosphoramidite - (10-1992)

3-Dimethoxytrityloxy-2-(3-(5-hexynamido)propanamido)propyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1992

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Product Specifications

Formula:

C₄₂H₅₅N₄O₇P

M.W.:

758.88

F.W.:

318.26

CAS Number:

2084118-98-7

3-Dimethoxytrityloxy-2-(3-(5-hexynamido)propanamido)propyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5’ terminus or within the sequence, and a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group.

Details

Usage

Coupling: 3 minute coupling time recommended.
Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours|at 65°C, 17 hours at room temperature or AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65°C.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1992-02	0.25 g	.25grams	3.29	96.33	57.8	36.13	26.27	19.27	4.82
10-1992-90	100 µmol	.076grams	1	20	12	7.5	5.45	4	1
10-1992-95	50 µmol	.038grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1992-02	0.25 g	.25grams	4.92	0.07	92	57.5	41.82	5.75
10-1992-90	100 µmol	.076grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1992-95	50 µmol	.038grams	0.75	0.07	8.6	5.38	3.91	0.54