Azidobutyrate NHS Ester - (50-1904)
4-Azido-butan-1-oic acid N-Hydroxysuccinimide Ester
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50-1904
4-Azido-butan-1-oic acid N-Hydroxysuccinimide Ester
Description
Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence.
REFERENCES|(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.
Details
Usage
- Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt.
| Specifications | |
|---|---|
| Diluent | Anhydrous ACN, DMSO or DMF |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 2-3 days |
Technical documents
Certificate of analysis
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