5'-AquaPhluor® 639 CE Phosphoramidite

6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H- Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6- (N-hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl] [trifluoroacetylaminobutoxy]phosphoryl][oxy]-hexyl-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C62H82ClF3N6O11P2S
M.W.:
1273.82
F.W.:
968.43
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

AquaPhluor® 639 is a rhodamine dye with an excitation and emission in the far red of the visible spectrum and is a logical alternative to Cyanine 5. Like Cyanine 5, AP639 is relatively bright and exhibits sequence- and pH-independent fluorescence. Unlike Cyanine 5, AP639 also shows excellent chemical and photochemical stability as well as temperature-independent fluorescence. AP639 is available as the phosphoramidite and CPG for 5’ and 3’ labeling, respectively.

Details

Usage

  • Coupling: 3 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Intellectual Property

This product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from ELITechGroup. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). AquaPhluor®, Yakima Yellow®, Redmond Red® and Eclipse® are registered trademarks of ELITechGroup.

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-5926-95 50 µmol 0.06 0.5 3.33 2 1.25 0.91 0.67 0.17
10-5926-90 100 µmol 0.13 1 20 12 7.5 5.45 4 1
10-5926-02 0.25 g 0.25 1.96 52 31.2 19.5 14.18 10.4 2.6

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-5926-95E 50 µmol 0.06 0.75 0.07 8.6 5.38 3.91 0.54
10-5926-90E 100 µmol 0.13 1.5 0.07 23.6 14.75 10.73 1.48
10-5926-02E 0.25 g 0.25 2.93 0.07 52.2 32.63 23.73 3.26