Ac-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C48H64N7O9PSi
M.W.:
941.43
F.W.:
345.21
CAS Number:
944138-03-8
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3025-02 0.25 g .25grams 2.66 75.33 45.2 28.25 20.55 15.07 3.77
10-3025-05 0.5 g .5grams 5.31 163.67 98.2 61.38 44.64 32.73 8.18
10-3025-10 1.0 g 1grams 10.62 340.67 204.4 127.75 92.91 68.13 17.03

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3025-02 0.25 g .25grams 3.96 0.07 72.8 45.5 15.17 4.55
10-3025-05 0.5 g .5grams 7.93 0.07 152.2 95.13 31.71 9.51
10-3025-10 1.0 g 1grams 15.85 0.07 310.6 194.13 64.71 19.41