Ac-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:

C₄₈H₆₄N₇O₉PSi

M.W.:

941.43

F.W.:

345.21

CAS Number:

944138-03-8

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Technical Documents

Description

Details

Dilution/Coupling Data

Technical Documents

Safety Data Sheet

TBDMS-Protected RNA Guide

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 9.12: Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Glen Report 4-12: RNA Synthesis - Options for 2'-0H Protection

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minute coupling time recommended
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30°C, dry
Stability	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3025-02	0.25 g	.25grams	2.66	75.33	45.2	28.25	20.55	15.07	3.77
10-3025-05	0.5 g	.5grams	5.31	163.67	98.2	61.38	44.64	32.73	8.18
10-3025-10	1.0 g	1grams	10.62	340.67	204.4	127.75	92.91	68.13	17.03

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3025-02	0.25 g	.25grams	3.96	0.07	72.8	45.5	15.17	4.55
10-3025-05	0.5 g	.5grams	7.93	0.07	152.2	95.13	31.71	9.51
10-3025-10	1.0 g	1grams	15.85	0.07	310.6	194.13	64.71	19.41