Home Products Modification and Labeling Label Phosphoramidites and Supports Acridine Labeling of Oligonucleotides 3'-Acridine CPG

3'-Acridine CPG - (20-2973)

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-succinoyl-long chain alkylamino-CPG

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20-2973

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Product Specifications

F.W.:

450.86

Pore Size:

1000Å

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-succinoyl-long chain alkylamino-CPG

Description

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Details

Usage

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
Deprotection: Cleavage and deprotection should be carried out using a mild 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support, neutralize with 1.5 volumes of 2M TEAA and desalt.|Note: NaOH is not compatible with dmf protecting groups.