Home Products Modification and Labeling Label Phosphoramidites and Supports Acridine Labeling of Oligonucleotides Acridine Phosphoramidite

Acridine Phosphoramidite - (10-1973)

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

As low as $165.00

In stock

Only %1 left

default

10-1973

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₅₁H₆₀N₄O₆PCl

M.W.:

891.53

F.W.:

450.86

CAS Number:

402829-33-8

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite

Description

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Details

Usage

Coupling: 15 minute coupling time is recommended.|(Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
Deprotection: Cleavage and deprotection should be carried out using a mild 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.|Note: NaOH is not compatible with dmf protecting groups.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1973-02	0.25 g	.25grams	2.8	80	48	30	21.82	16	4
10-1973-90	100 µmol	.089grams	1	20	12	7.5	5.45	4	1
10-1973-95	50 µmol	.045grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1973-02	0.25 g	.25grams	4.19	0.07	77.4	48.38	35.18	4.84
10-1973-90	100 µmol	.089grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1973-95	50 µmol	.045grams	0.75	0.07	8.6	5.38	3.91	0.54