Spacer Phosphoramidite 18 - (10-1918)
18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-1918
18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Description
The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers.
Details
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1918-02 | 0.25 g | .25grams | 3.18 | 92.67 | 55.6 | 34.75 | 25.27 | 18.53 | 4.63 |
| 10-1918-90 | 100 µmol | .078grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1918-02 | 0.25 g | .25grams | 4.75 | 0.07 | 88.6 | 55.38 | 40.27 | 5.54 |
| 10-1918-90 | 100 µmol | .078grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
Technical documents
Certificate of analysis
CoA search tool
Related products
Related Products
Spacer C12 CE Phosphoramidite
Cat. No.: 10-1928
dSpacer CE Phosphoramidite
Cat. No.: 10-1914
Spacer Phosphoramidite 9
Cat. No.: 10-1909
Spacer Phosphoramidite C3
Cat. No.: 10-1913
3'-Spacer C3 CPG
Cat. No.: 20-2913
PC Spacer Phosphoramidite
Cat. No.: 10-4913
References
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