What is the oligonucleotide synthesis scale for the Glen Gel-Pak™ desalting columns?
The oligonucleotide synthesis scale for the Glen Gel-Pak™ desalting columns is based on the oligonucleotide loading volume (mL), rather than the oligonucleotide synthesis scale (μmol). However, these two metrics are equivalent. Therefore:
Relevant products:
Glen Gel-Pak™ 1.0 Desalting Column (61-5010)
Glen Gel-Pak™ 2.5 Desalting Column (61-5025)
Glen Gel-Pak™ 0.2 Desalting Column (61-5002)
How does the HAA buffer compare to the TEAA buffer in my HPLC?
A solution of HAA (0.1M) behaves differently than TEAA at the same concentration. HAA is comprised of hexyl ammonium acetate and TEAA is made up of triethylammonium acetate. Hexylamine has a 6-carbon chain and the longer alkyl group interacts more strongly with reverse phase resins. As a result, HAA provides higher resolving power and is often preferred for DMT-OFF purifications.
HAA has a different and higher absorbance profile at 254 nm than TEAA. This is normal and should not interfere with your analysis. We have observed anywhere from 0-0.035 AU at 254 nm.
Relevant products:
