Glen Report 36-27: Technical Snippets

What is the oligonucleotide synthesis scale for the Glen Gel-Pak™ desalting columns? 

The oligonucleotide synthesis scale for the Glen Gel-Pak™ desalting columns is based on the oligonucleotide loading volume (mL), rather than the oligonucleotide synthesis scale (μmol). However, these two metrics are equivalent. Therefore:

0.2  Column = 0.2 μmol

1.0  Column = 1.0 μmol

2.5  Column =  2.5 μmol

Relevant products:

Glen Gel-Pak™  1.0 Desalting Column (61-5010)

Glen Gel-Pak™  2.5 Desalting Column (61-5025)

Glen Gel-Pak™  0.2 Desalting Column (61-5002)

How does the HAA buffer compare to the TEAA buffer in my HPLC?

A solution of HAA (0.1M) behaves differently than TEAA at the same concentration. HAA is comprised of hexyl ammonium acetate and TEAA is made up of triethylammonium acetate. Hexylamine has a 6-carbon chain and the longer alkyl group interacts more strongly with reverse phase resins. As a result, HAA provides higher resolving power and is often preferred for DMT-OFF purifications. 

HAA has a different and higher absorbance profile at 254 nm than TEAA. This is normal and should not interfere with your analysis. We have observed anywhere from 0-0.035 AU at 254 nm. 

Relevant products:

2.0M Hexylammonium Acetate, HPLC grade, pH=7 (60-4210)

2.0M Triethylamine Acetate, HPLC grade, pH=7 (60-4110)