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Ferrocene-dT-CE Phosphoramidite - (10-1576)

5'-dimethoxytrityloxy-5-[N-acetyl ferrocenyl-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1576

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Product Specifications

Formula:

C₆₀H₇₃FeN₆O₁₀P

M.W.:

1125.07

F.W.:

684.45

CAS Number:

2698331-21-2

5'-dimethoxytrityloxy-5-[N-acetyl ferrocenyl-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labelling to generate probes for electrochemical detection. Based on our Amino-Modifier C6-dT structure, Ferrocene-dT is easily added to oligonucleotides with no disruption of regular hybridization behavior. Multiple incorporations into an oligonucleotide probe are also simply achieved. Oligonucleotides are deprotected using standard techniques. Ferrocene oligonucleotides should be stored under Argon and aqueous solutions should be degassed immediately.

Details

Usage

Coupling: 3 minute coupling time recommended
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR Synthesize using UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx, Cap Mix A (40-4210-xx/ 40-4212-xx) and deprotect in Ammonium Hydroxide for 2 hours at room temperature.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1576-02	0.25 g	.25grams	2.22	60.67	36.4	22.75	16.55	12.13	3.03
10-1576-90	100 µmol	.113grams	1	20	12	7.5	5.45	4	1
10-1576-95	50 µmol	.056grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1576-02	0.25 g	.25grams	3.32	0.07	60	37.5	27.27	3.75
10-1576-90	100 µmol	.113grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1576-95	50 µmol	.056grams	0.75	0.07	8.6	5.38	3.91	0.54