Home Products Modification and Labeling Label Phosphoramidites and Supports EDTA Labeling of Oligonucleotides EDTA-C2-dT-CE Phosphoramidite

EDTA-C2-dT-CE Phosphoramidite - (10-1059)

5'-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1059

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Product Specifications

Formula:

C₆₀H₈₁N₈O₁₆P

M.W.:

1201.32

F.W.:

676.53

5'-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

EDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA. The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature.

Details

Usage

Coupling: 6 minute coupling time recommended.
Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.|Note: NaOH is not compatible with dmf protecting groups.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1059-02	0.25 g	.25grams	2.08	56	33.6	21	15.27	11.2	2.8
10-1059-90	100 µmol	.12grams	1	20	12	7.5	5.45	4	1
10-1059-95	50 µmol	.06grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1059-02	0.25 g	.25grams	3.11	0.07	55.8	34.88	25.36	3.49
10-1059-90	100 µmol	.12grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1059-95	50 µmol	.06grams	0.75	0.07	8.6	5.38	3.91	0.54