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dW-CE Phosphoramidite - (10-1527)

3-Methyl-2-pivaloyloxy-5-[2'-deoxy-3'-O-(2-cyanoethyl-N,N-diisopropylamino)-phosphino-5'-O-(dimethoxytrityl)-beta-D-ribofuranos-1'-yl]-6-(triisopropylsilylethynyl)-pyridine

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10-1527

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Product Specifications

Formula:

C₅₇H₇₈N₃O₈PSi

M.W.:

992.3

F.W.:

311.23

CAS Number:

2417413-14-8

3-Methyl-2-pivaloyloxy-5-[2'-deoxy-3'-O-(2-cyanoethyl-N,N-diisopropylamino)-phosphino-5'-O-(dimethoxytrityl)-beta-D-ribofuranos-1'-yl]-6-(triisopropylsilylethynyl)-pyridine

Description

dW is a C-nucleoside that acts as a strong adenine base pairing analog. In addition to the typical two hydrogen bonds found between T and A, dW can also interact with A via van der Waals forces. The result is a dW–A interaction that approaches the strength of a C–G base pair while also exhibiting enhanced base-pairing fidelity. dW can be used in place of T as a single substitution or a complete replacement for oligonucleotide hybridization applications. Please see Glen Report article 31.21 and Glen Report article 31.22 for more details.

Details

Usage

Coupling: A 15 minute coupling time is recommended. Deblocking should be carried out with 3% dichloroacetic acid in dichloromethane.
Deprotection: Deprotection must be carried out in the absence of heat. Otherwise, all standard conditions should be compatible. Typical conditions would be ammonium hydroxide or ammonium hydroxide/aqueous methylamine 1:1 at room temperature.
TIPS removal: After deprotection, dry down oligo and take up in 0.5 mL DMF. Add 0.1 mL TBAF. Vortex and keep at room temperature for 30 minutes. Mix with 0.5 mL 2 M TEAA and desalt on Glen Gel-Pak™ column or equivalent.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1527-02	0.25 g	.25grams	2.52	70.67	42.4	26.5	19.27	14.13	3.53
10-1527-90	100 µmol	.099grams	1	20	12	7.5	5.45	4	1
10-1527-95	50 µmol	.05grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1527-02	0.25 g	.25grams	3.76	0.07	68.8	43	31.27	4.3
10-1527-90	100 µmol	.099grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1527-95	50 µmol	.05grams	0.75	0.07	8.6	5.38	3.91	0.54