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8-oxo-dG-CE Phosphoramidite - (10-1028)

5'-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1028

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Product Specifications

Formula:

C₄₄H₅₄N₇O₉P

M.W.:

855.93

F.W.:

345.21

CAS Number:

143060-53-1

5'-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1028-02	0.25 g	.25grams	2.92	84	50.4	31.5	22.91	16.8	4.2
10-1028-90	100 µmol	.086grams	1	20	12	7.5	5.45	4	1
10-1028-95	50 µmol	.043grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1028-02	0.25 g	.25grams	4.36	0.07	80.8	50.5	36.73	5.05
10-1028-90	100 µmol	.086grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1028-95	50 µmol	.043grams	0.75	0.07	8.6	5.38	3.91	0.54