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dmf-5-Me-isodC-CE Phosphoramidite - (10-1065)

5'-Dimethoxytrityl-5-methyl-N-dimethylformamidine-2'-deoxyisoCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1065

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Product Specifications

Formula:

C₄₃H₅₅N₆O₇P

M.W.:

798.91

F.W.:

303.21

CAS Number:

242816-13-3

5'-Dimethoxytrityl-5-methyl-N-dimethylformamidine-2'-deoxyisoCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Unnatural base pairs display unique abilities in duplex DNA and in nucleic acid and protein biosyntheses. A standard Watson and Crick base pair is formed between iso-C and iso-G, but the hydrogen bonding pattern is quite different from the natural base pairs A-T and C-G. (The 5-methyl analogue was chosen as the synthetic target due to the reported instability of 2’-deoxyisocytidine caused by deamination during oligonucleotide synthesis or deprotection.)

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.|Note: Oligos containing 5-Me-iso-dC should be stored buffered at pH 7.5 to 8.0 to avoid decomposition.