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dmf-isodG-CE Phosphoramidite - (10-1078)

5'-Dimethoxytrityl-O2-diphenylcarbamoyl-N6-dimethylaminomethylidene-2'-deoxyisoGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1078

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Product Specifications

Formula:

C₅₆H₆₂N₉O₈P

M.W.:

1020.13

F.W.:

329.21

CAS Number:

915098-24-7

5'-Dimethoxytrityl-O2-diphenylcarbamoyl-N6-dimethylaminomethylidene-2'-deoxyisoGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Unnatural base pairs display unique abilities in duplex DNA and in nucleic acid and protein biosyntheses. A standard Watson and Crick base pair is formed between iso-C and iso-G, but the hydrogen bonding pattern is quite different from the natural base pairs A-T and C-G. (The 5-methyl analogue was chosen as the synthetic target due to the reported instability of 2’-deoxyisocytidine caused by deamination during oligonucleotide synthesis or deprotection.)

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.|Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.