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2',3'-ddA-CE Phosphoramidite - (10-7001)

N6-diisobutylaminomethylidene-2',3'-dideoxyAdenosine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-7001

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Product Specifications

Formula:

C₂₈H₄₇N₈O₃P

M.W.:

574.7

F.W.:

297.21

N6-diisobutylaminomethylidene-2',3'-dideoxyAdenosine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

To avoid polymerase extension at the 3' terminus, 2',3'-dideoxynucleoside and 3'-deoxynucleoside CPGs have proved to be effective. 2',3'- Phosphoramidites are designed to be used with the 5'-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3'-OH) groups are removed. (3'-Termination can also be effected using a 3'-3' linkage formed using 5'-supports, or 3'-spacer C3 CPG.)

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: 17 hours in Ammonium Hydroxide at 55°C or 1 hour at 65°C in Ammonium Hydroxide/40% Methylamine 1:1 (v/v) (AMA).