5'-Amino-dT-CE Phosphoramidite - (10-1932)
5'-monomethoxytritylamino-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-1932
5'-monomethoxytritylamino-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Description
5'-Amino-dT terminates an oligonucleotide with a 5'-amino group which may be used for attaching a peptide or a PNA sequence.
Details
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: Deprotect as required by nucleobases.WARNING: As with other trityl-protected amines, drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_MMT_Amino_Modifiers.pdf).
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability In Solution | 24 hours |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1932-02 | 0.25 g | .25grams | 3.5 | 103.33 | 62 | 38.75 | 28.18 | 20.67 | 5.17 |
| 10-1932-90 | 100 µmol | .071grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1932-02 | 0.25 g | .25grams | 5.23 | 0.07 | 98.2 | 61.38 | 44.64 | 6.14 |
| 10-1932-90 | 100 µmol | .071grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
Technical documents
Certificate of analysis
CoA search tool
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