5'-Amino-Modifier C3-TFA - (10-1923)
3-(Trifluoroacetylamino)propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramiditeGlen Research offers a range of different 5’-Amino-Modifiers designed for use in automated synthesizers to functionalize the 5’-terminus of a target oligonucleotide with a primary amine. These modifiers vary based on the amine protecting group and the linker. Of these, the trifluoroacetyl (TFA)-protected Amino-Modifiers are typically used when a trityl group is not necessary. The TFA group allows deprotection to be completed in a single standard step to give an amine that is immediately available for conjugation with amine-reactive agents.
The 5’-Amino-Modifier C3-TFA has a 3-carbon linker with a TFA protecting group.
It should be noted that TFA-amino modifiers are sensitive to prolonged transit times and elevated temperatures (e.g., international shipments in the summer). Under these conditions, PDA-amino modifiers are significantly more stable and are excellent alternatives (https://www.glenresearch.com/reports/gr25-16).
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications | |
---|---|
Diluent | Anhydrous Acetonitrile |
Recommended Storage | Freezer storage, -10 to -30°C, dry |
Stability In Solution | 24 hours |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-1923-02 | 0.25 g | .25grams | 6.73 | 211 | 126.6 | 79.13 | 57.55 | 42.2 | 10.55 |
10-1923-90 | 100 µmol | .037grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-1923-02 | 0.25 g | .25grams | 10.05 | 0.07 | 194.6 | 121.63 | 88.45 | 12.16 |
10-1923-90 | 100 µmol | .037grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
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