Alkyne-NHS Ester - (50-1905)
3-propargyloxypropanoic acid N-hydroxysuccinimidyl ester
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50-1905
3-propargyloxypropanoic acid N-hydroxysuccinimidyl ester
Description
Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5’ terminus or within the sequence, and a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group.
Details
Usage
- Coupling: For labeling 0.2µmoles of an amino-modified oligonucleotide, dissolve 2.3mg of the Alkyne-Modifier NHS Ester in 60µL of Acetonitrile (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500µL 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12µL of the Alkyne-Modifier NHS Ester solution and vortex. Let react for 2 hours at room temperature and desalt.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
Technical documents
Certificate of analysis
CoA search tool
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