Sulfurizing Reagent II

Sulfurizing Reagent II

Product Specifications

Formula:
C5H7N3S3
M.W.:
205.31
CAS Number:
1192027-04-5
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Sulfurizing Reagent II

Details

Usage

  • Coupling: Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
  • Deprotection: See TB_Sulfurizing II.pdf
Specifications
Diluent 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
Storage Controlled room temperature or lower, dry
Stability At least 6 months


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
40-4037-10 1 g 1grams 1 1 1 1 1 1 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
40-4037-10 1 g 1grams 1 1 1 1 1 1