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Ac-C-RNA-CPG - (20-3315)

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-acetate-3'-succinoyl-long chain alkylamino-CPG
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20-3315
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Product Specifications

F.W.:
305.18
Pore Size:
1000Å
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5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-acetate-3'-succinoyl-long chain alkylamino-CPG

Description

Glen Research offers RNA supports in which protected ribonucleosides are attached to CPG. With 5'-DMT protection, and all other protecting groups base-labile, the use of these supports is identical to DNA supports. These supports are suitable for use in producing oligodeoxynucleotides modified at the 3'-terminus or oligoribonucleotides.

Details

Usage

  • Coupling: Please refer to the technical bulletin for the synthesis and deprotection procedures. This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: Deprotect using the protocol required by the nucleobases.
Specifications
Recommended Storage Freezer storage, -10 to -30°C, dry
Technical documents
Safety Data Sheet (North America)
View Safety Data Sheet
Safety Data Sheet (EU)
View Safety Data Sheet
Safety Data Sheet (Other)
View Safety Data Sheet
TBDMS-Protected RNA Guide
View Bulletin
Glen Report 8.13: New Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers
View Report
Certificate of analysis

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Products FAQS
These supports (20-3303, 20-3315, 20-3324, 20-3330) can be used for oligodeoxynucleotide synthesis when a ribose functionality is desired at the 3'-terminus. Following periodate oxidation of the 1,2-diol, labelling or carrier molecules containing amino groups can be conjugated to the 3'-terminus. An example (1) is the use of poly-lysine as a carrier molecule.These supports can be used for RNA synthesis, and our other RNA supports (now all containing 2'-acetyl protecting groups, which are completely base-labile) can also be used for DNA modification.||REFERENCE(S):(1a) M. Lemaitre, B. Bayard, and B. Lebleu, Proc. Natl. Acad. Sci. USA, 1987, 84, 648-652., (1b) M. Lemaitre, C. Bisbal, B. Bayard, and B. Lebleu, Nucleosides & Nucleotides, 1987, 6, 311-315.