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5-Me-U-2'-MOE-Phosphoramidite - (10-3231)

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-uridine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3231

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Product Specifications

Formula:

C₄₃H₅₅N₄O₁₀P

M.W.:

818.9

F.W.:

378.27

CAS Number:

163878-63-5

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-uridine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3231-05	0.5 g	.5grams	6.11	190.33	114.2	71.38	51.91	38.07	9.52
10-3231-10	1.0 g	1grams	12.21	393.67	236.2	147.63	107.36	78.73	19.68
10-3231-20	2.0 g	2grams	24.42	800.67	480.4	300.25	218.36	160.13	40.03