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5-Me-C-2'-MOE-Phosphoramidite - (10-3211)

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl- cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3211

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Product Specifications

Formula:

C₅₀H₆₀N₅O₁₀P

M.W.:

922.03

F.W.:

377.29

CAS Number:

163759-94-2

5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl- cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.

Details

Usage

Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in a 4-5% 5-methyl-N4-methyl-C side product at the modification site.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3211-05	0.5 g	0.5grams	5.42	167.33	100.4	62.75	45.64	33.47	8.37
10-3211-10	1.0 g	1grams	10.85	348.33	209	130.63	95	69.67	17.42
10-3211-20	2.0 g	2grams	21.69	709.67	425.8	266.13	193.55	141.93	35.48