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2-Aminopurine-TBDMS-CE Phosphoramidite - (10-3070)

5'-Dimethoxytrityl-N2-isobutyryl-2-aminopurine riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite

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10-3070

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Product Specifications

Formula:

C₅₀H₆₈N₇O₈PSi

M.W.:

954.19

F.W.:

329.21

CAS Number:

151059-65-3

5'-Dimethoxytrityl-N2-isobutyryl-2-aminopurine riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite

2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Coupling: 12 minute coupling time recommended. See Technical Bulletin
Deprotection: RNA deprotaction using Ammonium Hydroxide:MethylAmine (AMA) 1:1 at 65°C for 10 minutes. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3070-02	0.25 g	.25grams	2.62	74	44.4	27.75	20.18	14.8	3.7
10-3070-90	100 µmol	.095grams	1	20	12	7.5	5.45	4	1
10-3070-95	50 µmol	.048grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3070-02	0.25 g	.25grams	3.91	0.07	71.8	44.8	14.96	4.4
10-3070-90	100 µmol	.095grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3070-95	50 µmol	.048grams	0.75	0.07	8.6	5.3	1.79	0.5