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PseudoUridine-CE Phosphoramidite - (10-3055)

5'-Dimethoxytrityl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3055

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Product Specifications

Formula:

C₄₅H₆₁N₄O₉PSi

M.W.:

861.05

F.W.:

306.17

CAS Number:

163496-23-9

5'-Dimethoxytrityl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

PseudoUridine is one of the most common modified nucleosides found in RNA. The availability of a phosphoramidite will allow detailed research into the effects of this modified base on RNA structure and activity.

Details

Usage

Coupling: 12 minute coupling time recommended.
Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3055-02	0.25 g	.25grams	2.9	83.33	50	31.25	22.73	16.67	4.17
10-3055-90	100 µmol	.086grams	1	20	12	7.5	5.45	4	1
10-3055-95	50 µmol	.043grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3055-02	0.25 g	.25grams	4.33	0.07	80.2	50.1	16.71	5
10-3055-90	100 µmol	.086grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3055-95	50 µmol	.043grams	0.75	0.07	8.6	5.3	1.79	0.5