3-Dimethoxytrityloxy-2-(3-(5-hexynamido)propanamido)propyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5’ terminus or within the sequence, and a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group.
Specifications | |
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Diluent | Anhydrous Acetonitrile |
Storage | Freezer storage, -10 to -30°C, dry |
Stability | 1-2 days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-1992-02 | 0.25 g | .25grams | 3.29 | 96.33 | 57.8 | 36.13 | 26.27 | 19.27 | 4.82 |
10-1992-90 | 100 µmol | .076grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1992-95 | 50 µmol | .038grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-1992-02 | 0.25 g | .25grams | 4.92 | 0.07 | 92 | 57.5 | 41.82 | 5.75 |
10-1992-90 | 100 µmol | .076grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
10-1992-95 | 50 µmol | .038grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
REFERENCES|(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.|(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.