Home Products Modification and Labeling Oligonucleotide Modification Reagents Amino-Modifiers 5'-Amino-Modifiers Universal-CE Phosphoramidite

Universal-CE Phosphoramidite - (10-5000)

3-[(3aR,4R, 5R, 6S, 7S)-6-(4,4'-dimethoxytrityloxy)-5-acetoxy-1-,3-dioxooctahydro-1H-4,7-epoxyisoindol-2-yl]propyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

As low as $45.00

In stock

Only %1 left

default

10-5000

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₄₃H₅₂N₃O₁₀P

M.W.:

801.87

CAS Number:

730963-37-8

3-[(3aR,4R, 5R, 6S, 7S)-6-(4,4'-dimethoxytrityloxy)-5-acetoxy-1-,3-dioxooctahydro-1H-4,7-epoxyisoindol-2-yl]propyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Cleavable linkers have a wide range of application uses. We now offer Universal-CE, a dual-purpose linker capable of revealing 3’-OH termini or serving as a 5’-amino modifier C3 upon cleavage. Used as a universal phosphoramidite, the resulting oligonucleotides with a 3’-OH may be crucial for downstream applications, such as primer extension or ligation assays. Used as an amino modifier, the resulting oligonucleotides would be identical to those generated with 5’-Amino-Modifier C3-TFA.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect using Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65 °C or Ammonium Hydroxide for 8 hours at 55 °C. For sensitive minor bases or dyes, use 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60 °C.