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Dabcyl-dT - (10-1058)

5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1058

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Product Specifications

Formula:

C₆₃H₇₆N₉O₁₀P

M.W.:

1150.32

F.W.:

709.7

CAS Number:

316121-62-7

5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3'-Dabsyl CPG and 3'-Dabcyl CPG are used to prepare probes with the quencher blocking the 3'-terminus. 5'-Dabcyl Phosphoramidite locates the quencher at the 5'-terminus and Dabcyl-dT places it within the sequence, leaving the 3'-terminus available for polymerase extension.

Details

Usage

Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). |A 6 minute coupling time is recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	10% THF in Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1058-02	0.25 g	.25grams	2.17	59	35.4	22.13	16.09	11.8	2.95
10-1058-90	100 µmol	.115grams	1	20	12	7.5	5.45	4	1
10-1058-95	50 µmol	.058grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1058-02	0.25 g	.25grams	3.24	0.07	58.4	36.5	26.55	3.65
10-1058-90	100 µmol	.115grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1058-95	50 µmol	.058grams	0.75	0.07	8.6	5.38	3.91	0.54