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NPOM Caged-dT-CE Phosphoramidite - (10-1534)

5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1534

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Product Specifications

Formula:

C₅₀H₅₈N₅O₁₃P

M.W.:

967.99

F.W.:

527.38 (N-POM-dT) FW: 304.20 (dT)

CAS Number:

942218-71-5

5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Photocaged oligonucleotides allow for the spatial temporal control of biological processes. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to non-invasive light that the native oligonucleotide is released.

Glen Research’s interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage. The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365 nm. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry. Light sensitive material.
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1534-02	0.25 g	.25grams	2.58	72.67	43.6	27.25	19.82	14.53	3.63
10-1534-90	100 µmol	.097grams	1	20	12	7.5	5.45	4	1
10-1534-95	50 µmol	.048grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1534-02	0.25 g	.25grams	3.85	0.07	70.6	44.13	32.09	4.41
10-1534-90	100 µmol	.097grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1534-95	50 µmol	.048grams	0.75	0.07	8.6	5.38	3.91	0.54