Home Products DNA & RNA Nucleosides, Analogs, and Supports Natural RNA Phosphoramidites and Supports Standard RNA Phosphoramidites and Supports TOM-protected phosphoramidites and supports for RNA synthesis TOM-Protected Phosphoramidites for RNA synthesis G-TOM-CE Phosphoramidite

G-TOM-CE Phosphoramidite - (10-3024)

5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

As low as $75.00

In stock

Only %1 left

default

10-3024

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₅₂H₇₂N₇O₁₀PSi

M.W.:

1014.24

F.W.:

345.21

CAS Number:

253586-14-0

5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

Coupling: See technical bulletin for details.
Deprotection: See technical bulletin for details.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3024-02	0.25 g	.25grams	2.46	68.67	41.2	25.75	18.73	13.73	3.43
10-3024-05	0.5 g	.5grams	4.93	151	90.6	56.63	41.18	30.2	7.55
10-3024-10	1.0 g	1grams	9.86	315.33	189.2	118.25	86	63.07	15.77

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3024-02	0.25 g	.25grams	3.68	0.07	67.2	42	14	4.2
10-3024-05	0.5 g	.5grams	7.36	0.07	140.8	88	29.33	8.8
10-3024-10	1.0 g	1grams	14.72	0.07	288	180	60	18