DNP-TEG Phosphoramidite - (10-1985)
1-Dimethoxytrityloxy-3-O-[N-(2,4-dinitrophenyl)-3-N-aminopropyl-(triethyleneglycol)]-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
As low as
$165.00
In stock
Only %1 left
default
10-1985
1-Dimethoxytrityloxy-3-O-[N-(2,4-dinitrophenyl)-3-N-aminopropyl-(triethyleneglycol)]-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Description
An analytical test based on detection of 2,4-dinitrophenyl (DNP) labelled oligonucleotides with anti-DNP antibodies has been proposed. We have chosen the branched triethylene glycol (TEG) spacer in our version of DNP phosphoramidite since it can be added once or several times to the 3' or 5' terminus.
Details
Usage
- Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on.
- Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with acetonitrile. At this point, the DMT group may be safely removed without loss of label during deprotection.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
| Stability In Solution | 2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1985-02 | 0.25 g | .25grams | 2.63 | 74.33 | 44.6 | 27.88 | 20.27 | 14.87 | 3.72 |
| 10-1985-90 | 100 µmol | .095grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1985-95 | 50 µmol | .048grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1985-02 | 0.25 g | .25grams | 3.93 | 0.07 | 72.2 | 45.13 | 32.82 | 4.51 |
| 10-1985-90 | 100 µmol | .095grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1985-95 | 50 µmol | .048grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
Technical documents
Certificate of analysis
CoA search tool
Related products
Related Products
Biotin-dT
Cat. No.: 10-1038
BiotinTEG Phosphoramidite
Cat. No.: 10-1955
5-Br-dU-CE Phosphoramidite
Cat. No.: 10-1090
Cholesteryl-TEG Phosphoramidite
Cat. No.: 10-1975
6-Fluorescein Phosphoramidite
Cat. No.: 10-1964
Products FAQS
Modification reagents based on a 1,2-diol, e.g., BioTEG, DNP phosphoramidites, or a 1,3-diol, e.g., fluorescein, biotin phosphoramidites, can be added several times at the 5'-terminus since they contain an alcohol group capable of further addition with phosphoramidites. Can this alcohol also be a substrate for T4 polynucleotide kinase for 32P labelling of these modified oligonucleotides? Surprisingly, the answer is yes. Teoule and coworkers have shown(1) that oligos labelled at the 5'-terminus are substrates for kinase. Interestingly, the oligos modified with reagents based on 1,2-diols are labelled to 50%, indicating that only one diastereomer is labelled, while those modified with 1,3-diol reagents are labelled to 100%.||REFERENCE(S): (1) M.L. Fontanel, H. Bazin, and R. Teoule, Analytical Biochemistry, 1993, 214, 338-340. ||
To request a quote for products:
- Click “Contact Us” in the header bar above;
- Click “Customer Service”;
- Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
- Click “Submit”.