dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C47H52N7O7P
M.W.:
857.95
F.W.:
313.21
CAS Number:
98796-53-3
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet (North America)

Safety Data Sheet (EU)

Safety Data Sheet (Other)

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 28.14: New Product - Depurination Resistant dA-CE Phosphoramidite

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination, and each vial type is thoroughly tested or inspected to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1000-05 0.5 g .5grams 5.83 181 108.6 67.88 49.36 36.2 9.05
10-1000-10 1.0 g 1grams 11.66 375.33 225.2 140.75 102.36 75.07 18.77
10-1000-20 2.0 g 2grams 23.31 763.67 458.2 286.38 208.27 152.73 38.18
10-1000-40 4.0 g 4grams 46.62 1540.67 924.4 577.75 420.18 308.13 77.03

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1000-C2 0.25 g .25grams 4.35 0.07 83.8 52.38 38.09 5.24
10-1000-C5 0.5 g .5grams 8.7 0.07 170.8 106.75 77.64 10.68
10-1000-1C 1.0 g 1grams 17.4 0.07 344.8 215.5 156.73 21.55
10-1000-2C 2.0 g 2grams 34.79 0.07 692.6 432.88 314.82 43.29

References

Generation of high-affinity DNA aptamers using an expanded genetic alphabet Michiko Kimoto, Rie Yamashige, Ken-ichiro Matsunaga, Shigeyuki Yokoyama & Ichiro Hirao

Development of decoy oligonucleotide-warheaded chimeric molecules targeting STAT3 Po-Chang Shih, Miyako Naganuma, Genichiro Tsuji, Yosuke Demizu, and Mikihiko Naito

A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E.coli RNA polymerase Juntaek Oh, Zelin Shan, Shuichi Hoshika, Jun Xu, Jenny Chong, Steven A. Benner, Dmitry Lyumkis, and Dong Wang

Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds Wamhoff, EC., Ronsard, L., Feldman, J. et al.

A multiplexed and quantitative assay for small-molecule detection via photo-crosslinking of structure switching aptamers Sharon S. Newman, Brandon Wilson, Liwei Zheng, Michael Eisenstein, Tom Soh

Selection of DNA aptamers that prevent the fibrillization of a-synuclein protein in cellular and mouse models Erin M. McConnell, Dennis Chan, Katelyn Ventura, Joshua P. Callahan, Kathryn Harris, Vernon H. Hunt, Spencer Boisjoli, Daniel Knight, Evan T. Monk, Matthew R. Holahan, and Maria C. DeRose.

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