Some of our more popular minor base and modifier supports are available on polystyrene in columns fully compatible with the Applied Biosystems 3900 synthesizer. These include our popular Universal Support III, which will allow DNA, RNA or LNA oligos to be produced on the 3900 with ANY base at the 3’ terminus. At the same time, we are offering 1 μmole columns of Universal Support III for the 3900 instrument. Structures and more complete descriptions are found in the relevant catalog sections for each item. ABI 3900 columns can be prepared with virtually any of the CPG supports in our catalog.
It is no longer necessary to adjust the flow using our ABI 3900 CPG columns. Modified CPG columns are only available in 200 nmole size - simple add ‘A’ to the regular catalog number to order.
ABI 3900 1000Å CPG COLUMNS
Glen Research’s ABI 3900 1000Å CPG columns bring the lower cost of CPG to this platform while maintaining the high synthesis efficiency of 1000Å CPG. Our columns offer the following key attributes:
No need to change instrument settings
No need to change software parameters
Easier handling post -synthesis compared to PS
High quality 1000Å CPG for optimal synthesis results
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STERLING CE PHOSPHORAMIDITES
Glen Research CE (β-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.
STERLING SUPPORTS
All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
STERLING CE PHOSPHORAMIDITES
is a trademark of Glen Research Corporation.