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A-TOM-CE Phosphoramidite - (10-3004)

5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3004

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Product Specifications

Formula:

C₅₂H₇₂N₇O₉PSi

M.W.:

998.24

F.W.:

329.21

CAS Number:

253586-13-9

5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

Coupling: See technical bulletin for details.
Deprotection: See technical bulletin for details.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3004-02	0.25 g	.25grams	2.5	70	42	26.25	19.09	14	3.5
10-3004-05	0.5 g	.5grams	5.01	153.67	92.2	57.63	41.91	30.73	7.68
10-3004-10	1.0 g	1grams	10.02	320.67	192.4	120.25	87.45	64.13	16.03

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3004-02	0.25 g	.25grams	3.74	0.07	68	42	14.25	4
10-3004-05	0.5 g	.5grams	7.48	0.07	143	89	29.83	8
10-3004-10	1.0 g	1grams	14.95	0.07	292	182	60.96	18